Answering Your Scientific Questions

Each analysis module in MetaboNexus is a specialized tool designed to answer a specific biological question. Explore our comprehensive suite of methods.

UMAP Plot

Dimensionality Reduction

Get a high-level "map" of your data's structure. Do your experimental groups form distinct clusters? Is there unexpected heterogeneity? PCA, UMAP & t-SNE can tell you.

 LISA Plot

Spatial Autocorrelation

Is your metabolite's distribution random or spatially structured? Use Moran's I for a global score and LISA to find the exact location of significant hotspots and coldspots.

Leiden Clustering Plot

Leiden Clustering

Perform unsupervised spatial segmentation to discover distinct metabolic regions in your tissue, revealing hidden biological structures and tumor microenvironments.

 Correlation Heatmap

Correlation Analysis

Understand systemic metabolic relationships by calculating the pairwise correlation for every metabolite, hinting at shared biological pathways and regulatory mechanisms.

 Enrichment Analysis Plot

Enrichment Analysis

Connect molecular observations to biological function. Find out if your list of interesting metabolites are statistically over-represented in known biological pathways.